Ontology highlight
ABSTRACT:
SUBMITTER: Uchtenhagen H
PROVIDER: S-EPMC5013714 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Uchtenhagen Hannes H Rims Cliff C Blahnik Gabriele G Chow I-Ting IT Kwok William W WW Buckner Jane H JH James Eddie A EA
Nature communications 20160830
MHC tetramers are an essential tool for characterizing antigen-specific CD4+ T cells. However, their ex vivo analysis is limited by the large sample requirements. Here we demonstrate a combinatorial staining approach that allows simultaneous characterization of multiple specificities to address this challenge. As proof of principle, we analyse CD4+ T-cell responses to the seasonal influenza vaccine, establishing a frequency hierarchy and examining differences in memory and activation status, lin ...[more]