Unknown

Dataset Information

0

MiR-21 promotes intrahepatic cholangiocarcinoma proliferation and growth in vitro and in vivo by targeting PTPN14 and PTEN.


ABSTRACT: Intrahepatic cholangiocarcinoma (ICC) constitutes the second-most common primary hepatic malignancy. MicroRNAs (miRNAs) play important roles in the pathogenesis of ICC. However, the clinical significance of miR-21 levels in ICC remains unclear. Here, we investigated the role of miR-21 in ICC and found that its expression was significantly upregulated in serum of ICC patients. Serum miR-21 levels robustly distinguished ICC patients from control subjects. Further experiments showed that inhibition of miR-21 suppressed ICC cell proliferation in vitro and tumor growth in vivo. Specifically, inhibition of miR-21 induced cell cycle arrest and apoptosis. Moreover, PTPN14 and PTEN were identified as direct and functional targets of miR-21. Finally, we showed high expression levels of miR-21 were closely related to adverse clinical features, diminished survival, and poor prognosis in ICC patients. This study revealed functional and mechanistic links between miR-21 and tumor suppressor genes, PTPN14 and PTEN, in the pathogenesis of ICC. MiR-21 not only plays important roles in the regulation of cell proliferation and tumor growth in ICC, but is also a diagnostic and prognostic marker, and a potential therapeutic target for ICC.

SUBMITTER: Wang LJ 

PROVIDER: S-EPMC4467412 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications

MiR-21 promotes intrahepatic cholangiocarcinoma proliferation and growth in vitro and in vivo by targeting PTPN14 and PTEN.

Wang Li-Juan LJ   He Chen-Chen CC   Sui Xin X   Cai Meng-Jiao MJ   Zhou Cong-Ya CY   Ma Jin-Lu JL   Wu Lei L   Wang Hao H   Han Su-Xia SX   Zhu Qing Q  

Oncotarget 20150301 8


Intrahepatic cholangiocarcinoma (ICC) constitutes the second-most common primary hepatic malignancy. MicroRNAs (miRNAs) play important roles in the pathogenesis of ICC. However, the clinical significance of miR-21 levels in ICC remains unclear. Here, we investigated the role of miR-21 in ICC and found that its expression was significantly upregulated in serum of ICC patients. Serum miR-21 levels robustly distinguished ICC patients from control subjects. Further experiments showed that inhibition  ...[more]

Similar Datasets

| S-EPMC4147955 | biostudies-literature
| S-EPMC6416795 | biostudies-literature
| S-EPMC3975380 | biostudies-literature
| S-EPMC2427229 | biostudies-literature
| S-EPMC5289448 | biostudies-literature
| S-EPMC6643209 | biostudies-literature
| S-EPMC4118890 | biostudies-literature
| S-EPMC3107097 | biostudies-literature
| S-EPMC6549920 | biostudies-literature
| S-EPMC6249286 | biostudies-literature