Ontology highlight
ABSTRACT:
SUBMITTER: Ferguson ML
PROVIDER: S-EPMC4469183 | biostudies-literature | 2006 May
REPOSITORIES: biostudies-literature
Ferguson Matthew L ML Prasad Kondury K Sackett Dan L DL Boukari Hacène H Lafer Eileen M EM Nossal Ralph R
Biochemistry 20060501 18
A principal component in the protein coats of certain post-golgi and endocytic vesicles is clathrin, which appears as a three-legged heteropolymer (known as a triskelion) that assembles into polyhedral cages principally made up of pentagonal and hexagonal faces. In vitro, this assembly depends upon the pH, with cages forming more readily at low pH and less readily at high pH. We have developed procedures, on the basis of static and dynamic light scattering, to determine the radius of gyration, R ...[more]