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Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide.

ABSTRACT: A biotinylated heparosan hexasaccharide was synthesized using a one-pot multi-enzyme strategy, in situ activation and transfer of N-trifluoroacetylglucosamine (GlcNTFA) to a heparin backbone significantly improved the synthetic efficiency. The biotinylated hexasaccharide could serve as a flexible core to diversify its conversion into heparan sulfate isoforms with potential biological applications and therapeutics.

SUBMITTER: Wu B

PROVIDER: S-EPMC4472006 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature

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Facile chemoenzymatic synthesis of biotinylated heparosan hexasaccharide.

Wu Baolin B Wei Na N Thon Vireak V Wei Mohui M Yu Zaikuan Z Xu Yongmei Y Chen Xi X Liu Jian J Wang Peng George PG Li Tiehai T

Organic & biomolecular chemistry 20150501 18

A biotinylated heparosan hexasaccharide was synthesized using a one-pot multi-enzyme strategy, in situ activation and transfer of N-trifluoroacetylglucosamine (GlcNTFA) to a heparin backbone significantly improved the synthetic efficiency. The biotinylated hexasaccharide could serve as a flexible core to diversify its conversion into heparan sulfate isoforms with potential biological applications and therapeutics. ...[more]

PMID: 25858766

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