Ontology highlight
ABSTRACT:
SUBMITTER: de Lorenzo S
PROVIDER: S-EPMC4472040 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
de Lorenzo Sara S Ribezzi-Crivellari Marco M Arias-Gonzalez J Ricardo JR Smith Steven B SB Ritort Felix F
Biophysical journal 20150601 12
To our knowledge, we have developed a novel temperature-jump optical tweezers setup that changes the temperature locally and rapidly. It uses a heating laser with a wavelength that is highly absorbed by water so it can cover a broad range of temperatures. This instrument can record several force-distance curves for one individual molecule at various temperatures with good thermal and mechanical stability. Our design has features to reduce convection and baseline shifts, which have troubled previ ...[more]