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Rapid endosomal escape of prickly nanodiamonds: implications for gene delivery.

ABSTRACT: The prickly nanodiamonds easily entered cells via endocytosis followed by unique intracellular translocation characteristics—quick endosomal escape followed by stable residence in cytoplasm. Endosomal membrane rupturing is identified as the major route of nanodiamonds' escaping the vesicle confinement and to the cytoplasm. Little cytotoxicity is observed to associate with the nanodiamonds' cytosolic release. Such features enable its application for gene delivery, which requires both effective cellular uptake and cytosolic release of the gene. Taking green fluorescent protein gene as an example, we demonstrate the successful cytosolic delivery and expression of such a gene using the prickly nanodiamonds as carrier.

SUBMITTER: Chu Z

PROVIDER: S-EPMC4485068 | biostudies-literature | 2015 Jun

REPOSITORIES: biostudies-literature

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Rapid endosomal escape of prickly nanodiamonds: implications for gene delivery.

Chu Zhiqin Z Miu Kaikei K Lung Pingsai P Zhang Silu S Zhao Saisai S Chang Huan-Cheng HC Lin Ge G Li Quan Q

Scientific reports 20150630

The prickly nanodiamonds easily entered cells via endocytosis followed by unique intracellular translocation characteristics—quick endosomal escape followed by stable residence in cytoplasm. Endosomal membrane rupturing is identified as the major route of nanodiamonds' escaping the vesicle confinement and to the cytoplasm. Little cytotoxicity is observed to associate with the nanodiamonds' cytosolic release. Such features enable its application for gene delivery, which requires both effective ce ...[more]

PMID: 26123532

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