Ontology highlight
ABSTRACT:
SUBMITTER: Watanabe S
PROVIDER: S-EPMC4485131 | biostudies-literature | 2015 Jun
REPOSITORIES: biostudies-literature
Watanabe Satoshi S Kawashima Takumi T Nishitani Yuichi Y Kanai Tamotsu T Wada Takehiko T Inaba Kenji K Atomi Haruyuki H Imanaka Tadayuki T Miki Kunio K
Proceedings of the National Academy of Sciences of the United States of America 20150608 25
The Ni atom at the catalytic center of [NiFe] hydrogenases is incorporated by a Ni-metallochaperone, HypA, and a GTPase/ATPase, HypB. We report the crystal structures of the transient complex formed between HypA and ATPase-type HypB (HypBAT) with Ni ions. Transient association between HypA and HypBAT is controlled by the ATP hydrolysis cycle of HypBAT, which is accelerated by HypA. Only the ATP-bound form of HypBAT can interact with HypA and induces drastic conformational changes of HypA. Conseq ...[more]