Ontology highlight
ABSTRACT:
SUBMITTER: Kwon S
PROVIDER: S-EPMC6142260 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Kwon Sunghark S Watanabe Satoshi S Nishitani Yuichi Y Kawashima Takumi T Kanai Tamotsu T Atomi Haruyuki H Miki Kunio K
Proceedings of the National Academy of Sciences of the United States of America 20180618 27
Ni-Fe clusters are inserted into the large subunit of [NiFe] hydrogenases by maturation proteins such as the Ni chaperone HypA via an unknown mechanism. We determined crystal structures of an immature large subunit HyhL complexed with HypA from <i>Thermococcus kodakarensis</i> Structure analysis revealed that the N-terminal region of HyhL extends outwards and interacts with the Ni-binding domain of HypA. Intriguingly, the C-terminal extension of immature HyhL, which is cleaved in the mature form ...[more]