Application of BRET to monitor ligand binding to GPCRs.
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ABSTRACT:
SUBMITTER: Stoddart LA
PROVIDER: S-EPMC4488387 | biostudies-literature | 2015 Jul
REPOSITORIES: biostudies-literature
Publications
Application of BRET to monitor ligand binding to GPCRs.
Stoddart Leigh A LA Johnstone Elizabeth K M EKM Wheal Amanda J AJ Goulding Joëlle J Robers Matthew B MB Machleidt Thomas T Wood Keith V KV Hill Stephen J SJ Pfleger Kevin D G KDG
Nature methods 20150601 7
Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein-coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs. ...[more]
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