Ontology highlight
ABSTRACT:
SUBMITTER: Thirukkumaran OM
PROVIDER: S-EPMC6970966 | biostudies-literature | 2019
REPOSITORIES: biostudies-literature
Thirukkumaran Ovia Margaret OM Wang Congrong C Asouzu Nnamdi Joseph NJ Fron Eduard E Rocha Susana S Hofkens Johan J Lavis Luke D LD Mizuno Hideaki H
Frontiers in chemistry 20200114
Bioluminescence resonance energy transfer (BRET) from an exceptionally bright luciferase, NanoLuc, to a fluorescent HaloTag ligand is gaining momentum to monitor molecular interactions. The recommended use of HaloTag618 ligand for the NanoLuc-HaloTag BRET pair is versatile for ensemble experiments due to their well-separated emission bands. However, this system is not applicable for single-cell BRET imaging because of its low BRET efficiency and in turn weak acceptor signals. Here we explored th ...[more]