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Design and synthesis of active heparan sulfate-based probes.

ABSTRACT: A chemoenzymatic approach for synthesizing heparan sulfate oligosaccharides with a reactive diazoacetyl saccharide residue is reported. The resultant oligosaccharides were demonstrated to serve as specific inhibitors for heparan sulfate sulfotransferases, offering a new set of tools to probe the structural selectivity for heparan sulfate-binding proteins.

SUBMITTER: Zhou W

PROVIDER: S-EPMC4502442 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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Design and synthesis of active heparan sulfate-based probes.

Zhou Wen W Hsieh Po-Hung PH Xu Yongmei Y O'Leary Timothy R TR Huang Xuefei X Liu Jian J

Chemical communications (Cambridge, England) 20150701 55

A chemoenzymatic approach for synthesizing heparan sulfate oligosaccharides with a reactive diazoacetyl saccharide residue is reported. The resultant oligosaccharides were demonstrated to serve as specific inhibitors for heparan sulfate sulfotransferases, offering a new set of tools to probe the structural selectivity for heparan sulfate-binding proteins. ...[more]

PMID: 26066846

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