Unknown

Dataset Information

0

Highly polymorphic microsatellite markers in Pulsatilla vulgaris (Ranunculaceae) using next-generation sequencing.

ABSTRACT: PREMISE OF THE STUDY:We developed novel microsatellite markers for the perennial plant Pulsatilla vulgaris (Ranunculaceae) to investigate the effects of fragmentation on gene flow in this imperiled species. METHODS AND RESULTS:We identified microsatellites and developed primers based on 454 shotgun sequences. We identified 14 markers that were polymorphic and produced clean bands. Of these, eight could be analyzed as diploids. Genotyping of 97 individuals across two populations revealed these markers to be highly polymorphic with seven to 17 alleles per locus and observed heterozygosity from 0.41 to 0.83. CONCLUSIONS:The markers are highly informative and will be used to test if the reintroduction of shepherding in southern Germany improves genetic connectivity among fragmented populations of P. vulgaris. The combination of diploid and tetraploid markers presented here will be useful in resolving the polyploidization history of this and related species.

SUBMITTER: DiLeo MF 

PROVIDER: S-EPMC4504725 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

Highly polymorphic microsatellite markers in Pulsatilla vulgaris (Ranunculaceae) using next-generation sequencing.

DiLeo Michelle F MF   Graf René R   Holderegger Rolf R   Rico Yessica Y   Wagner Helene H HH  

Applications in plant sciences 20150707 7

<h4>Premise of the study</h4>We developed novel microsatellite markers for the perennial plant Pulsatilla vulgaris (Ranunculaceae) to investigate the effects of fragmentation on gene flow in this imperiled species.<h4>Methods and results</h4>We identified microsatellites and developed primers based on 454 shotgun sequences. We identified 14 markers that were polymorphic and produced clean bands. Of these, eight could be analyzed as diploids. Genotyping of 97 individuals across two populations re  ...[more]

Similar Datasets

Unknown Comparative genome-wide polymorphic microsatellite markers in Antarctic penguins through next generation sequencing.
| S-EPMC5596379 | biostudies-literature
Unknown Development of Novel Polymorphic Microsatellite Markers in Catch Bowl Coral, Isopora palifera (Scleractinia; Acroporidae) Using Next-generation Sequencing.
| S-EPMC6517735 | biostudies-literature
Unknown Development of microsatellite markers for Sargentodoxa cuneata (Lardizabalaceae) using next-generation sequencing technology.
| S-EPMC4873275 | biostudies-literature
Unknown Development of microsatellite markers for the endangered Pedicularis ishidoyana (Orobanchaceae) using next-generation sequencing.
| S-EPMC4683043 | biostudies-literature
Unknown Characterization of the complete mitochondrial genome and a set of polymorphic microsatellite markers through next-generation sequencing for the brown brocket deer Mazama gouazoubira.
| S-EPMC4612593 | biostudies-literature
Unknown Development of 14 microsatellite markers in the Queensland koala (Phascolarctos cinereus adustus) using next generation sequencing technology.
| S-EPMC4109682 | biostudies-other
Unknown Applicability of next generation sequencing technology in microsatellite instability testing.
| S-EPMC4377833 | biostudies-other
Unknown Development of microsatellite markers for the Korean Mussel, Mytilus coruscus (Mytilidae) using next-generation sequencing.
| S-EPMC3431879 | biostudies-literature
Genomics Polymorphic microsatellite markers for Microbotryum saponariae
| PRJEB11435 | ENA
Unknown Development of 17 novel microsatellite markers for Lycoris aurea and L. radiata (Amaryllidaceae) using next-generation sequencing.
| S-EPMC6240451 | biostudies-literature