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Development of 17 microsatellite markers in the federally endangered species Liatris helleri (Asteraceae).

ABSTRACT: Premise:Microsatellite markers were developed in the federally endangered species Liatris helleri (Asteraceae) to evaluate species boundaries with closely related congeners within the genus. Methods and Results:Using Illumina data, 17 primer pairs were developed in populations of L. helleri. The primers amplified motifs from tri- to hexanucleotide repeats with one to 17 alleles per locus. Primers were also tested for cross-amplification in L. aspera, L. microcephala, and L. pycnostachya. Conclusions:The developed primers for L. helleri serve as a novel genetic tool for future investigations in this genus, allowing for more explicit species delineation as well as population genetic analyses.

SUBMITTER: Clark LC

PROVIDER: S-EPMC6636620 | biostudies-literature | 2019 Jul

REPOSITORIES: biostudies-literature

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Development of 17 microsatellite markers in the federally endangered species <i>Liatris helleri</i> (Asteraceae).

Clark Logan C LC Gaglianese-Woody Morgan R MR Estep Matt C MC

Applications in plant sciences 20190517 7

<h4>Premise</h4>Microsatellite markers were developed in the federally endangered species <i>Liatris helleri</i> (Asteraceae) to evaluate species boundaries with closely related congeners within the genus.<h4>Methods and results</h4>Using Illumina data, 17 primer pairs were developed in populations of <i>L. helleri</i>. The primers amplified motifs from tri- to hexanucleotide repeats with one to 17 alleles per locus. Primers were also tested for cross-amplification in <i>L. aspera</i>, <i>L. mic ...[more]

PMID: 31346504

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Correction to Development and characterization of microsatellite primers in the federally endangered <i>Astragalus bibullatus</i> (Fabaceae).

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