Dataset Information

Accumulation of extra-chloroplastic triacylglycerols in Arabidopsis seedlings during heat acclimation.

ABSTRACT: Heat acclimation enables plants to tolerate and survive short-term heat stress on hot days. In Arabidopsis thaliana, a genetically programmed heat shock response can be rapidly triggered in the temperature range of 32-38°C through activation of heat shock transcription factors (HSF). The heat shock response leads to heat acclimation and confers short-term protection against temperatures above 40°C. However, little is known about metabolic adjustments during heat acclimation.Untargeted metabolite analyses of A. thaliana seedlings revealed that levels of polyunsaturated triacylglycerols (TG) rapidly and dramatically increase during heat acclimation. TG accumulation was found to be temperature dependent in a temperature range of 32-50°C (optimum at 42°C) and reversible after a return from 37°C to normal growth temperatures. Heat-induced TGs accumulated in extra-chloroplastic compartments and increased in both roots and shoots to a similar extent. Analysis of mutants deficient in all four HSFA1 master regulator genes or the HSFA2 gene revealed that TG accumulation was not dependent on HSFs. Moreover, the TG response was not limited to heat stress because drought and salt stress also triggered an accumulation of TGs, but not short-term osmotic, cold, and high light stress. Lipid analysis revealed that heat-induced accumulation of TGs was not due to massive de novo fatty acid synthesis. It is hypothesized that TGs serve as transient stores for fatty acids that may be required for membrane remodelling during heat acclimation.

SUBMITTER: Mueller SP

PROVIDER: S-EPMC4507766 | biostudies-literature | 2015 Aug

REPOSITORIES: biostudies-literature

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Accumulation of extra-chloroplastic triacylglycerols in Arabidopsis seedlings during heat acclimation.

Mueller Stephanie P SP Krause Daniel M DM Mueller Martin J MJ Fekete Agnes A

Journal of experimental botany 20150514 15

Heat acclimation enables plants to tolerate and survive short-term heat stress on hot days. In Arabidopsis thaliana, a genetically programmed heat shock response can be rapidly triggered in the temperature range of 32-38°C through activation of heat shock transcription factors (HSF). The heat shock response leads to heat acclimation and confers short-term protection against temperatures above 40°C. However, little is known about metabolic adjustments during heat acclimation.Untargeted metabolite ...[more]

PMID: 25977236

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Project description:In order to pinpoint phytohormone changes associated with enhanced heat stress tolerance, the complex phytohormone profiles [cytokinins, auxin, abscisic acid (ABA), jasmonic acid (JA), salicylic acid and ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC)] were compared in Arabidopsis thaliana after direct heat shock (45°C, 3 h) and in heat-stressed pre-acclimated plants (1 h at 37°C followed by 2 h at optimal temperature 20°C). Organ-specific responses were followed in shoot apices, leaves, and roots immediately after heat shock and after 24-h recovery at 20°C. The stress strength was evaluated via membrane ion leakage and the activity of nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (NOX) and antioxidant enzymes [superoxide dismutases, guaiacol peroxidases (POD), catalases, ascorbate peroxidases (APX)]. Heat acclimation diminished negative effects of heat stress, especially in apices and roots, no significant differences being observed in leaves. Low NOX1-3 activities indicated diminished production of reactive oxygen species. Higher activity of APX, POD1, and the occurrence of POD3-4 reflected acclimation-stimulated readiness of the antioxidant system. Acclimation diminished heat shock-induced changes of ABA, JA, cytokinin, and auxin levels in apices. Excess of ABA catabolites suggested an early stress response. The strong up-regulation of ABA and ACC in roots indicated defense boost in roots of acclimated plants compared to the non-acclimated ones. To evaluate the possibility to enhance stress tolerance by cytokinin pool modulation, INCYDE-F, an inhibitor of cytokinin oxidase/dehydrogenase, was applied. As cytokinin effects on stress tolerance may depend on timing of their regulation, INCYDE was applied at several time-points. In combination with acclimation, INCYDE treatment had a slight positive effect on heat stress tolerance, mainly when applied after 2-h period of the optimal temperature. INCYDE increased contents of cytokinins trans-zeatin and cis-zeatin in roots and auxin in all tissues after heat shock. INCYDE also helped to suppress the content of ABA in leaves, and ethylene in apices and roots. INCYDE application to non-acclimated plants (applied before or after heat shock) strengthened negative stress effects, probably by delaying of the repair processes. In conclusion, pre-treatment with moderately elevated temperature enhanced heat stress tolerance and accelerated recovery after stress. Inhibition of cytokinin degradation by INCYDE slightly improved recovery of acclimated plants.

Project description:Overwintering plants are capable of exhibiting high levels of cold tolerance, which is acquired through the process of cold acclimation (CA). In contrast to CA, the acquired freezing tolerance is rapidly reduced during cold de-acclimation (DA) and plants resume growth after sensing warm temperatures. In order to better understand plant growth and development, and to aid in the breeding of cold-tolerant plants, it is important to decipher the functional mechanisms of the DA process. In this study, we performed comparative transcriptomic and proteomic analyses during CA and DA. As revealed by shotgun proteomics, we identified 3987 peptides originating from 1569 unique proteins and the corresponding mRNAs were analyzed. Among the 1569 genes, 658 genes were specifically induced at the transcriptional level during the process of cold acclimation. In order to investigate the relationship between mRNA and the corresponding protein expression pattern, a Pearson correlation was analyzed. Interestingly, 199 genes showed a positive correlation of mRNA and protein expression pattern, indicating that both their transcription and translation occurred during CA. However, 226 genes showed a negative correlation of mRNA and protein expression pattern, indicating that their mRNAs were transcribed during CA and were stored for the subsequent DA step. Under this scenario, those proteins were specifically increased during DA without additional transcription of mRNA. In order to confirm the negative correlation of mRNA and protein expression patterns, qRT-PCR and western blot analyses were performed. Mitochondrial malate dehydrogenase 1 (mMDH1) exhibited a negative correlation of mRNA and protein levels, which was characterized by CA-specific mRNA induction and protein accumulation specifically during DA. These data indicate that the expression of specific mRNAs and subsequent accumulation of corresponding proteins are not always in accordance under low temperature stress conditions in plants.

Dataset Information

Accumulation of extra-chloroplastic triacylglycerols in Arabidopsis seedlings during heat acclimation.

Publications

Accumulation of extra-chloroplastic triacylglycerols in Arabidopsis seedlings during heat acclimation.

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