Unknown

Dataset Information

0

A Novel Role of E-Cadherin-Based Adherens Junctions in Neoplastic Cell Dissemination.


ABSTRACT: Using confocal microscopy, we analyzed the behavior of IAR-6-1, IAR1170, and IAR1162 transformed epithelial cells seeded onto the confluent monolayer of normal IAR-2 epithelial cells. Live-cell imaging of neoplastic cells stably expressing EGFP and of normal epithelial cells stably expressing mKate2 showed that transformed cells retaining expression of E-cadherin were able to migrate over the IAR-2 epithelial monolayer and invade the monolayer. Transformed IAR cells invaded the IAR-2 monolayer at the boundaries between normal cells. Studying interactions of IAR-6-1 transformed cells stably expressing GFP-E-cadherin with the IAR-2 epithelial monolayer, we found that IAR-6-1 cells established E-cadherin-based adhesions with normal epithelial cells: dot-like dynamic E-cadherin-based adhesions in protrusions and large adherens junctions at the cell sides and rear. A comparative study of a panel of transformed IAR cells that differ by their ability to form E-cadherin-based AJs, either through loss of E-cadherin expression or through expression of a dominant negative E-cadherin mutant, demonstrated that E-cadherin-based AJs are key mediators of the interactions between neoplastic and normal epithelial cells. IAR-6-1DNE cells expressing a dominant-negative mutant form of E-cadherin with the mutation in the first extracellular domain practically lost the ability to adhere to IAR-2 cells and invade the IAR-2 epithelial monolayer. The ability of cancer cells to form E-cadherin-based AJs with the surrounding normal epithelial cells may play an important role in driving cancer cell dissemination in the body.

SUBMITTER: Rubtsova SN 

PROVIDER: S-EPMC4514802 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

altmetric image

Publications

A Novel Role of E-Cadherin-Based Adherens Junctions in Neoplastic Cell Dissemination.

Rubtsova Svetlana N SN   Zhitnyak Irina Y IY   Gloushankova Natalya A NA  

PloS one 20150724 7


Using confocal microscopy, we analyzed the behavior of IAR-6-1, IAR1170, and IAR1162 transformed epithelial cells seeded onto the confluent monolayer of normal IAR-2 epithelial cells. Live-cell imaging of neoplastic cells stably expressing EGFP and of normal epithelial cells stably expressing mKate2 showed that transformed cells retaining expression of E-cadherin were able to migrate over the IAR-2 epithelial monolayer and invade the monolayer. Transformed IAR cells invaded the IAR-2 monolayer a  ...[more]

Similar Datasets

| S-EPMC363079 | biostudies-other
| S-EPMC3251172 | biostudies-literature
| S-EPMC5411698 | biostudies-literature
| S-EPMC4457789 | biostudies-literature
| S-EPMC2779654 | biostudies-literature
| S-EPMC5948979 | biostudies-literature
| S-EPMC2678428 | biostudies-literature
| S-EPMC4418904 | biostudies-literature
| S-EPMC6510885 | biostudies-literature
| S-EPMC3847041 | biostudies-literature