Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging.
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ABSTRACT:
SUBMITTER: Ong WQ
PROVIDER: S-EPMC4544551 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Publications
Heavy water: a simple solution to increasing the brightness of fluorescent proteins in super-resolution imaging.
Ong Wei Qiang WQ Citron Y Rose YR Schnitzbauer Joerg J Kamiyama Daichi D Huang Bo B
Chemical communications (Cambridge, England) 20150901 70
Photoactivatable fluorescent proteins (PA-FPs) are widely used in live single-molecule super-resolution imaging but emit substantially fewer photons than organic dyes do. Herein, we show that in heavy water (D2O) instead of H2O, common PA-FPs emit 26-54% more photons, effectively improving the localization precision in super-resolution imaging. ...[more]
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