Ontology highlight
ABSTRACT:
SUBMITTER: Nilson KA
PROVIDER: S-EPMC4546572 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Nilson Kyle A KA Guo Jiannan J Turek Michael E ME Brogie John E JE Delaney Elizabeth E Luse Donal S DS Price David H DH
Molecular cell 20150806 4
The Cdk7 subunit of TFIIH phosphorylates RNA polymerase II (Pol II) during initiation, and, while recent studies show that inhibition of human Cdk7 negatively influences transcription, the mechanisms involved are unclear. Using in vitro transcription with nuclear extract, we demonstrate that THZ1, a covalent Cdk7 inhibitor, causes defects in Pol II phosphorylation, co-transcriptional capping, promoter proximal pausing, and productive elongation. THZ1 does not affect initiation but blocks essenti ...[more]