Ontology highlight
ABSTRACT:
SUBMITTER: Hosios AM
PROVIDER: S-EPMC4548833 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Hosios Aaron M AM Fiske Brian P BP Gui Dan Y DY Vander Heiden Matthew G MG
Molecular cell 20150820 5
The role of pyruvate kinase M2 (PKM2) in cell proliferation is controversial. A unique function of PKM2 proposed to be important for the proliferation of some cancer cells involves the direct activity of this enzyme as a protein kinase; however, a detailed biochemical characterization of this activity is lacking. Using [(32)P]-phosphoenolpyruvate (PEP) we examine the direct substrates of PKM2 using recombinant enzyme and in vitro systems where PKM2 is genetically deleted. Labeling of some protei ...[more]