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Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells.

ABSTRACT: We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (?90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.

SUBMITTER: Nair SV

PROVIDER: S-EPMC4552044 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature

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Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells.

Nair Soumya V SV Witek Małgorzata A MA Jackson Joshua M JM Lindell Maria A M MA Hunsucker Sally A SA Sapp Travis T Perry Caroline E CE Hupert Mateusz L ML Bae-Jump Victoria V Gehrig Paola A PA Wysham Weiya Z WZ Armistead Paul M PM Voorhees Peter P Soper Steven A SA

Chemical communications (Cambridge, England) 20150201 15

We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (∼90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved. ...[more]

PMID: 25616078

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