Dataset Information
Lentivirus-Mediated siRNA Targeting ER-? Inhibits Tumorigenesis and Induces Apoptosis in Hepatocarcinoma Cells.
Ontology highlight
ABSTRACT:
Methods: Lentivirus-mediated ER-? small interfering RNA (siRNA) was transfected into HCC cells Hep3B. ER-? expression was monitored by real-time polymerase chain reaction (PCR) and western blot. Cell proliferation, apoptosis, and invasion were examined by methyl thiazol tetrazolium (MTT), flow cytometry (FCM), and invasion assay, respectively.
Results: ER-? siRNA efficiently downregulated the expression of ER-? in Hep3B cells at both mRNA and protein levels in a time-dependent manner. ER-? siRNA also inhibited cell proliferation and reduced cell invasion (compared with other groups, P < 0.05, resp.). Furthermore, knockdown of ER-? slowed down the cell population at S phase and increased the rate of apoptosis (P < 0.05, resp.).
Conclusion: ER-? knockdown suppressed the growth of HCC cells. Thus, ER-? may play a very important role in carcinogenesis of HCC and its knockdown may offer a new potential gene therapy approach for human liver cancer in the future.
SUBMITTER: Jiang P
PROVIDER: S-EPMC4564599 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Publications
Lentivirus-Mediated siRNA Targeting ER-α Inhibits Tumorigenesis and Induces Apoptosis in Hepatocarcinoma Cells.
BioMed research international 20150827
<h4>Background and objectives</h4>Estrogen receptor-α (ER-α) plays important roles in hepatocarcinogenesis. Recent studies have shown that ER-α could lead to cell cycle progression or inhibition of apoptosis. To better understand the role of ER-α, RNA interference (RNAi) was used to inhibit ER-α expression in the human hepatocellular carcinoma (HCC) cells.<h4>Methods</h4>Lentivirus-mediated ER-α small interfering RNA (siRNA) was transfected into HCC cells Hep3B. ER-α expression was monitored by ...[more]
