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Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction.

ABSTRACT: Morphological and functional differences of the right and left ventricle are apparent in the adult human heart. A differential contribution of cardiac fibroblasts and smooth muscle cells (populations of epicardium-derived cells) to each ventricle may account for part of the morphological-functional disparity. Here we studied the relation between epicardial derivatives and the development of compact ventricular myocardium.Wildtype and Wt1CreERT2/+ reporter mice were used to study WT-1 expressing cells, and Tcf21lacZ/+ reporter mice and PDGFR?-/-;Tcf21LacZ/+ mice to study the formation of the cardiac fibroblast population. After covering the heart, intramyocardial WT-1+ cells were first observed at the inner curvature, the right ventricular postero-lateral wall and left ventricular apical wall. Later, WT-1+ cells were present in the walls of both ventricles, but significantly more pronounced in the left ventricle. Tcf21-LacZ + cells followed the same distribution pattern as WT-1+ cells but at later stages, indicating a timing difference between these cell populations. Within the right ventricle, WT-1+ and Tcf21-lacZ+ cell distribution was more pronounced in the posterior inlet part. A gradual increase in myocardial wall thickness was observed early in the left ventricle and at later stages in the right ventricle. PDGFR?-/-;Tcf21LacZ/+ mice showed deficient epicardium, diminished number of Tcf21-LacZ + cells and reduced ventricular compaction.During normal heart development, spatio-temporal differences in contribution of WT-1 and Tcf21-LacZ + cells to right versus left ventricular myocardium occur parallel to myocardial thickening. These findings may relate to lateralized differences in ventricular (patho)morphology in humans.

SUBMITTER: Vicente-Steijn R

PROVIDER: S-EPMC4577115 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction.

Vicente-Steijn Rebecca R Scherptong Roderick W C RW Kruithof Boudewijn P T BP Duim Sjoerd N SN Goumans Marie Jose T H MJ Wisse Lambertus J LJ Zhou Bin B Pu William T WT Poelmann Robert E RE Schalij Martin J MJ Tallquist Michelle D MD Gittenberger-de Groot Adriana C AC Jongbloed Monique R M MR

PloS one 20150921 9

<h4>Background</h4>Morphological and functional differences of the right and left ventricle are apparent in the adult human heart. A differential contribution of cardiac fibroblasts and smooth muscle cells (populations of epicardium-derived cells) to each ventricle may account for part of the morphological-functional disparity. Here we studied the relation between epicardial derivatives and the development of compact ventricular myocardium.<h4>Results</h4>Wildtype and Wt1CreERT2/+ reporter mice ...[more]

PMID: 26390289

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Dataset Information

Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction.

Publications

Regional differences in WT-1 and Tcf21 expression during ventricular development: implications for myocardial compaction.

Similar Datasets

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