Ontology highlight
ABSTRACT:
SUBMITTER: Lin L
PROVIDER: S-EPMC4583019 | biostudies-literature | 2015 Sep
REPOSITORIES: biostudies-literature
Lin Lin L Gopal Srila S Sharda Anish A Passam Freda F Bowley Sheryl R SR Stopa Jack J Xue Guangpu G Yuan Cai C Furie Barbara C BC Flaumenhaft Robert R Huang Mingdong M Furie Bruce B
The Journal of biological chemistry 20150803 39
Quercetin-3-rutinoside inhibits thrombus formation in a mouse model by inhibiting extracellular protein disulfide isomerase (PDI), an enzyme required for platelet thrombus formation and fibrin generation. Prior studies have identified PDI as a potential target for novel antithrombotic agents. Using a fluorescence enhancement-based assay and isothermal calorimetry, we show that quercetin-3-rutinoside directly binds to the b' domain of PDI with a 1:1 stoichiometry. The binding of quercetin-3-rutin ...[more]