EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy.
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ABSTRACT:
SUBMITTER: Barad BA
PROVIDER: S-EPMC4589481 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Publications
EMRinger: side chain-directed model and map validation for 3D cryo-electron microscopy.
Barad Benjamin A BA Echols Nathaniel N Wang Ray Yu-Ruei RY Cheng Yifan Y DiMaio Frank F Adams Paul D PD Fraser James S JS
Nature methods 20150817 10
Advances in high-resolution cryo-electron microscopy (cryo-EM) require the development of validation metrics to independently assess map quality and model geometry. We report EMRinger, a tool that assesses the precise fitting of an atomic model into the map during refinement and shows how radiation damage alters scattering from negatively charged amino acids. EMRinger (https://github.com/fraser-lab/EMRinger) will be useful for monitoring progress in resolving and modeling high-resolution feature ...[more]
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