?-Klotho Expression in Human Tissues.

ABSTRACT: CONTEXT:?-Klotho has emerged as a powerful regulator of the aging process. To date, the expression profile of ?-Klotho in human tissues is unknown, and its existence in some human tissue types is subject to much controversy. OBJECTIVE:This is the first study to characterize systemwide tissue expression of transmembrane ?-Klotho in humans. We have employed next-generation targeted proteomic analysis using parallel reaction monitoring in parallel with conventional antibody-based methods to determine the expression and spatial distribution of human ?-Klotho expression in health. RESULTS:The distribution of ?-Klotho in human tissues from various organ systems, including arterial, epithelial, endocrine, reproductive, and neuronal tissues, was first identified by immunohistochemistry. Kidney tissues showed strong ?-Klotho expression, whereas liver did not reveal a detectable signal. These results were next confirmed by Western blotting of both whole tissues and primary cells. To validate our antibody-based results, ?-Klotho-expressing tissues were subjected to parallel reaction monitoring mass spectrometry (data deposited at ProteomeXchange, PXD002775) identifying peptides specific for the full-length, transmembrane ?-Klotho isoform. CONCLUSIONS:The data presented confirm ?-Klotho expression in the kidney tubule and in the artery and provide evidence of ?-Klotho expression across organ systems and cell types that has not previously been described in humans.

SUBMITTER: Lim K 

PROVIDER: S-EPMC4596032 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature