Dataset Information

Extrarenal ?-klotho expression in human tissues

ABSTRACT: ?-Klotho has emerged as a powerful regulator of the ageing process. To-date, the expression profile of ?-Klotho in human tissues is unknown and its existence in some human tissue types is subject to much controversy. Objective: This is the first study to characterize system-wide tissue expression of transmembrane ?-Klotho in humans. We have employed next generation targeted proteomic analysis using Parallel Reaction Monitoring (PRM) in parallel with conventional antibody-based methods to determine the expression and spatial distribution of human ?-Klotho expression in health. Results: The distribution of ?-Klotho in human tissues from various organ systems, including arterial, epithelial, endocrine, reproductive and neuronal tissues was first identified by immunohistochemistry. Kidney tissues showed strong ?-Klotho expression, while liver did not reveal a detectable signal. These results were next confirmed by western blotting of both whole tissues and primary cells. To validate our antibody-based results, ?-Klotho expressing tissues were subjected to PRM mass spectrometry identifying peptides specific for the full length, transmembrane ?-Klotho isoform. Conclusions: The data presented confirms ?-Klotho expression in the kidney tubule and in artery, and provides evidence of ?-Klotho expression across organ systems and cell-types that have not previously been described in humans.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Kathryn Lilley

PROVIDER: MSV000080719 | MassIVE | Wed Mar 29 00:22:00 BST 2017

SECONDARY ACCESSION(S): PXD002775

REPOSITORIES: MassIVE

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α-Klotho Expression in Human Tissues.

Lim Kenneth K Groen Arnoud A Molostvov Guerman G Lu Tzongshi T Lilley Kathryn S KS Snead David D James Sean S Wilkinson Ian B IB Ting Stephen S Hsiao Li-Li LL Hiemstra Thomas F TF Zehnder Daniel D

The Journal of clinical endocrinology and metabolism 20150817 10

<h4>Context</h4>α-Klotho has emerged as a powerful regulator of the aging process. To date, the expression profile of α-Klotho in human tissues is unknown, and its existence in some human tissue types is subject to much controversy.<h4>Objective</h4>This is the first study to characterize systemwide tissue expression of transmembrane α-Klotho in humans. We have employed next-generation targeted proteomic analysis using parallel reaction monitoring in parallel with conventional antibody-based met ...[more]

PMID: 26280509

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