Dataset Information

Extrarenal ?-klotho expression in human tissues

ABSTRACT: ?-Klotho has emerged as a powerful regulator of the ageing process. To-date, the expression profile of ?-Klotho in human tissues is unknown and its existence in some human tissue types is subject to much controversy. Objective: This is the first study to characterize system-wide tissue expression of transmembrane ?-Klotho in humans. We have employed next generation targeted proteomic analysis using Parallel Reaction Monitoring (PRM) in parallel with conventional antibody-based methods to determine the expression and spatial distribution of human ?-Klotho expression in health. Results: The distribution of ?-Klotho in human tissues from various organ systems, including arterial, epithelial, endocrine, reproductive and neuronal tissues was first identified by immunohistochemistry. Kidney tissues showed strong ?-Klotho expression, while liver did not reveal a detectable signal. These results were next confirmed by western blotting of both whole tissues and primary cells. To validate our antibody-based results, ?-Klotho expressing tissues were subjected to PRM mass spectrometry identifying peptides specific for the full length, transmembrane ?-Klotho isoform. Conclusions: The data presented confirms ?-Klotho expression in the kidney tubule and in artery, and provides evidence of ?-Klotho expression across organ systems and cell-types that have not previously been described in humans.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (ncbitaxon:9606)

SUBMITTER: Kathryn Lilley

PROVIDER: MSV000080719 | MassIVE | Wed Mar 29 00:22:00 BST 2017

SECONDARY ACCESSION(S): PXD002775

REPOSITORIES: MassIVE

ACCESS DATA

Publications

α-Klotho Expression in Human Tissues.

Lim Kenneth K Groen Arnoud A Molostvov Guerman G Lu Tzongshi T Lilley Kathryn S KS Snead David D James Sean S Wilkinson Ian B IB Ting Stephen S Hsiao Li-Li LL Hiemstra Thomas F TF Zehnder Daniel D

The Journal of clinical endocrinology and metabolism 20150817 10

<h4>Context</h4>α-Klotho has emerged as a powerful regulator of the aging process. To date, the expression profile of α-Klotho in human tissues is unknown, and its existence in some human tissue types is subject to much controversy.<h4>Objective</h4>This is the first study to characterize systemwide tissue expression of transmembrane α-Klotho in humans. We have employed next-generation targeted proteomic analysis using parallel reaction monitoring in parallel with conventional antibody-based met ...[more]

PMID: 26280509

Similar Datasets

Project description:Uhlén2015 - Human tissue-based proteome metabolic network - kidney Human kidney specific proteome metabolic network This model is described in the article: Tissue-based map of the human proteome Uhlén M, Fagerberg L, Hallström BM, Lindskog C, Oksvold P, Mardinoglu A, Sivertsson Å, Kampf C, Sjöstedt E, Asplund A, Olsson I, Edllund K, Lundberg E, Navani S, Szigyarto AC, Odeberg J, Djureinovic D, Takanen JO, Hober S, Alm T, Edqvist P, Berlin H, Tegel H, Mulder J, Rockberg J, Nilsson P, Schwenk JM, Hamsten M, von Feilitzen K, Forsberg M, Persson L, Johansson F, Zwahlen M, von Heijne G, Nielsen J, Pontén F. Science 2015 January; 347(6220) Abstract: Resolving the molecular details of proteome variation in the different tissues and organs of the human body will greatly increase our knowledge of human biology and disease. Here, we present a map of the human tissue proteome based on an integrated omics approach that involves quantitative transcriptomics at the tissue and organ level, combined with tissue microarray–based immunohistochemistry, to achieve spatial localization of proteins down to the single-cell level. Our tissue-based analysis detected more than 90% of the putative protein-coding genes. We used this approach to explore the human secretome, the membrane proteome, the druggable proteome, the cancer proteome, and the metabolic functions in 32 different tissues and organs. All the data are integrated in an interactive Web-based database that allows exploration of individual proteins, as well as navigation of global expression patterns, in all major tissues and organs in the human body. This model is hosted on BioModels Database and identified by: MODEL1411240019. To cite BioModels Database, please use: BioModels: ten-year anniversary . To the extent possible under law, all copyright and related or neighbouring rights to this encoded model have been dedicated to the public domain worldwide. Please refer to CC0 Public Domain Dedication for more information.

Dataset Information

Extrarenal ?-klotho expression in human tissues

Publications

α-Klotho Expression in Human Tissues.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure