Ontology highlight
ABSTRACT:
SUBMITTER: Liu B
PROVIDER: S-EPMC4604464 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Scientific reports 20151014
We demonstrate the use of cryogenic super-resolution correlative light and electron microscopy (csCLEM) to precisely determine the spatial relationship between proteins and their native cellular structures. Several fluorescent proteins (FPs) were found to be photoswitchable and emitted far more photons under our cryogenic imaging condition, resulting in higher localization precision which is comparable to ambient super-resolution imaging. Vitrified specimens were prepared by high pressure freezi ...[more]