Ontology highlight
ABSTRACT:
SUBMITTER: Kocylowski MK
PROVIDER: S-EPMC4615105 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Cell cycle (Georgetown, Tex.) 20150101 11
The mammalian E3 ubiquitin ligases RNF8 and RNF168 facilitate recruitment of the DNA damage response protein 53BP1 to sites of DNA double-strand breaks (DSBs). The mechanism involves recruitment of RNF8, followed by recruitment of RNF168, which ubiquitinates histones H2A/H2AX on K15. 53BP1 then binds to nucleosomes at sites of DNA DSBs by recognizing, in addition to methyl marks, histone H2A/H2AX ubiquitinated on K15. We report here that expressing H2AX fusion proteins with N-terminal bulky moie ...[more]