Ontology highlight
ABSTRACT:
SUBMITTER: Honda S
PROVIDER: S-EPMC4615770 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
RNA biology 20150101 5
Transfer RNAs (tRNAs) play a central role in translation and also recently appear to have a variety of other functions in biological processes beyond translation. Here we report the development of Four-Leaf clover qRT-PCR (FL-PCR), a convenient PCR-based method, which can specifically quantify individual mature tRNA species. In FL-PCR, T4 RNA ligase 2 specifically ligates a stem-loop adapter to mature tRNAs but not to precursor tRNAs or tRNA fragments. Subsequent TaqMan qRT-PCR amplifies only un ...[more]