Ontology highlight
ABSTRACT:
SUBMITTER: Lykkemark S
PROVIDER: S-EPMC4622476 | biostudies-literature | 2014
REPOSITORIES: biostudies-literature
Lykkemark Simon S Mandrup Ole Aalund OA Friis Niels Anton NA Kristensen Peter P
mAbs 20140101 6
Expression of recombinant proteins often takes advantage of peptide tags expressed in fusion to allow easy detection and purification of the expressed proteins. However, as the fusion peptides most often are flexible appendages at the N- or C-terminal, proteolytic cleavage may result in removal of the tag sequence. Here, we evaluated the functionality and stability of 14 different combinations of commonly used tags for purification and detection of recombinant antibody fragments. The tag sequenc ...[more]