Ontology highlight
ABSTRACT:
SUBMITTER: Wu Y
PROVIDER: S-EPMC4630089 | biostudies-literature | 2015 Oct
REPOSITORIES: biostudies-literature
Wu Yong Y Wu Xundong X Toro Ligia L Stefani Enrico E
Methods (San Diego, Calif.) 20150627
STED (stimulated emission depletion) is a popular super-resolution fluorescence microscopy technique. In this paper, we present a concise guide to building a resonant-scanning STED microscope with ultrafast photon-counting acquisition. The STED microscope has two channels, using a pulsed laser and a continuous-wave (CW) laser as the depletion laser source, respectively. The CW STED channel preforms time-gated detection to enhance optical resolution in this channel. We use a resonant mirror to at ...[more]