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Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody.

ABSTRACT: Geographical expansion and re-emerging new genotypes of the Japanese encephalitis virus (JEV) require the development of novel therapeutic approaches. Here, we studied a non-conventional approach for antibody therapy and show that, upon exposure to heme, a fraction of natural human immunoglobulins acquires high-affinity reactivity with the antigenic domain-III of JEV E glycoprotein. These JEV-reactive antibodies exhibited neutralizing activity against recently dominant JEV genotypes. This study opens new therapeutic options for Japanese encephalitis.

SUBMITTER: Gupta N

PROVIDER: S-EPMC4635365 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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Neutralization of Japanese Encephalitis Virus by heme-induced broadly reactive human monoclonal antibody.

Gupta Nimesh N de Wispelaere Mélissanne M Lecerf Maxime M Kalia Manjula M Scheel Tobias T Vrati Sudhanshu S Berek Claudia C Kaveri Srinivas V SV Desprès Philippe P Lacroix-Desmazes Sébastien S Dimitrov Jordan D JD

Scientific reports 20151106

Geographical expansion and re-emerging new genotypes of the Japanese encephalitis virus (JEV) require the development of novel therapeutic approaches. Here, we studied a non-conventional approach for antibody therapy and show that, upon exposure to heme, a fraction of natural human immunoglobulins acquires high-affinity reactivity with the antigenic domain-III of JEV E glycoprotein. These JEV-reactive antibodies exhibited neutralizing activity against recently dominant JEV genotypes. This study ...[more]

PMID: 26542535

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