Ontology highlight
ABSTRACT:
SUBMITTER: Pechhold S
PROVIDER: S-EPMC4638177 | biostudies-literature | 2009 Nov
REPOSITORIES: biostudies-literature
Pechhold Susanne S Stouffer Melissa M Walker Gregory G Martel Ralph R Seligmann Bruce B Hang Yan Y Stein Roland R Harlan David M DM Pechhold Klaus K
Nature biotechnology 20091018 11
Analyzing specialized cells in heterogeneous tissues is crucial for understanding organ function in health and disease. Thus far, however, there has been no convenient method for studying gene expression in cells purified by fluorescence-activated cell sorting (FACS) using intracellular markers. Here we show that the quantitative nuclease protection assay (qNPA) enables transcriptional analysis of intracytoplasmically stained cells sorted by FACS. Applying the method to mouse pancreatic islet-ce ...[more]