Unknown

Dataset Information

0

Chromatin Immunoprecipitation of Low Number of FACS-Purified Epidermal Cells.


ABSTRACT: Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is a method designed to detect interactions between chromatin and the proteins bound to it. This method has been widely used for characterizing epigenetic landscapes in many cell types; however, a limiting factor has been the requirement of a high number of cells. Here, we describe a protocol for ChIP in epidermal cells from a newborn mouse, purified by fluorescence-activated cell sorting (FACS). This protocol has been optimized specifically for prefixed, low cell numbers, resulting in enough immunoprecipitated DNA suitable for genome-wide analysis.

SUBMITTER: Bar C 

PROVIDER: S-EPMC8278243 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

| S-EPMC9996616 | biostudies-literature
| S-EPMC5997839 | biostudies-literature
| S-EPMC4123551 | biostudies-literature
| S-EPMC9421536 | biostudies-literature
2016-12-09 | GSE76779 | GEO
| S-EPMC4638177 | biostudies-literature
| S-EPMC6653780 | biostudies-literature
| S-EPMC8092002 | biostudies-literature
| S-EPMC2832845 | biostudies-literature
| S-EPMC4268110 | biostudies-literature