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DNA-based identification of Calendula officinalis (Asteraceae).

ABSTRACT:

Premise of the study

For the economically important species Calendula officinalis, a fast identification assay based on high-resolution melting curve analysis was designed. This assay was developed to distinguish C. officinalis from other species of the genus and other Asteraceae genera, and to detect C. officinalis as an adulterant of saffron samples.

Methods and results

For this study, five markers (ITS, rbcL, 5' trnK-matK, psbA-trnH, trnL-trnF) of 10 Calendula species were sequenced and analyzed for species-specific mutations. With the application of two developed primer pairs located in the trnK 5' intron and trnL-trnF, C. officinalis could be distinguished from other species of the genus and all outgroup samples tested. Adulterations of Calendula DNA in saffron could be detected down to 0.01%.

Conclusions

With the developed assay, C. officinalis can be reliably identified and admixtures of this species as adulterant of saffron can be revealed at low levels.

SUBMITTER: Schmiderer C

PROVIDER: S-EPMC4651632 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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DNA-based identification of Calendula officinalis (Asteraceae).

Schmiderer Corinna C Lukas Brigitte B Ruzicka Joana J Novak Johannes J

Applications in plant sciences 20151103 11

<h4>Premise of the study</h4>For the economically important species Calendula officinalis, a fast identification assay based on high-resolution melting curve analysis was designed. This assay was developed to distinguish C. officinalis from other species of the genus and other Asteraceae genera, and to detect C. officinalis as an adulterant of saffron samples.<h4>Methods and results</h4>For this study, five markers (ITS, rbcL, 5' trnK-matK, psbA-trnH, trnL-trnF) of 10 Calendula species were sequ ...[more]

PMID: 26649268

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