Ontology highlight
ABSTRACT:
SUBMITTER: McDonald BD
PROVIDER: S-EPMC4654978 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Immunity 20151027 5
The precise impact of thymic positive and negative selection on the T cell receptor (TCR) repertoire remains controversial. Here, we used unbiased, high-throughput cloning and retroviral expression of individual pre-selection TCRs to provide a direct assessment of these processes at the clonal level in vivo. We found that 15% of random TCRs induced signaling and directed positive (7.5%) or negative (7.5%) selection, depending on strength of signal, whereas the remaining 85% failed to induce sign ...[more]