Ontology highlight
ABSTRACT:
SUBMITTER: Kelly PP
PROVIDER: S-EPMC4660908 | biostudies-literature | 2015
REPOSITORIES: biostudies-literature
Kelly Paul P PP Eichler Anja A Herter Susanne S Kranz David C DC Turner Nicholas J NJ Flitsch Sabine L SL
Beilstein journal of organic chemistry 20150922
Cytochrome P450 monooxygenases are useful biocatalysts for C-H activation, and there is a need to expand the range of these enzymes beyond what is naturally available. A panel of 93 variants of active self-sufficient P450cam[Tyr96Phe]-RhFRed fusion enzymes with a broad diversity in active site amino acids was developed by screening a large mutant library of 16,500 clones using a simple, highly sensitive colony-based colorimetric screen against indole. These mutants showed distinct fingerprints o ...[more]