Electroblotting from Polyacrylamide Gels.
Ontology highlight
ABSTRACT:
SUBMITTER: Goldman A
PROVIDER: S-EPMC4663689 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Publications
Electroblotting from Polyacrylamide Gels.
Goldman Aaron A Ursitti Jeanine A JA Mozdzanowski Jacek J Speicher David W DW
Current protocols in protein science 20151102
Transferring proteins from polyacrylamide gels onto retentive membranes is now primarily used for immunoblotting. A second application that was quite common up to about a decade ago was electroblotting of proteins for N-terminal and internal sequencing using Edman chemistry. This unit contains procedures for electroblotting proteins from polyacrylamide gels onto a variety of membranes, including polyvinylidene difluoride (PVDF) and nitrocellulose. In addition to the commonly used tank or wet tra ...[more]
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