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The electrophoresis of transferrins in urea/polyacrylamide gels.


ABSTRACT: The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss of iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to denature at different urea concentrations.

SUBMITTER: Evans RW 

PROVIDER: S-EPMC1162034 | biostudies-other | 1980 Sep

REPOSITORIES: biostudies-other

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