Ontology highlight
ABSTRACT:
SUBMITTER: Trivedi DV
PROVIDER: S-EPMC4664342 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Trivedi Darshan V DV Muretta Joseph M JM Swenson Anja M AM Davis Jonathon P JP Thomas David D DD Yengo Christopher M CM
Proceedings of the National Academy of Sciences of the United States of America 20151109 47
Myosins use a conserved structural mechanism to convert the energy from ATP hydrolysis into a large swing of the force-generating lever arm. The precise timing of the lever arm movement with respect to the steps in the actomyosin ATPase cycle has not been determined. We have developed a FRET system in myosin V that uses three donor-acceptor pairs to examine the kinetics of lever arm swing during the recovery and power stroke phases of the ATPase cycle. During the recovery stroke the lever arm sw ...[more]