Ontology highlight
ABSTRACT:
SUBMITTER: Kumar RP
PROVIDER: S-EPMC4682395 | biostudies-literature | 2015 Dec
REPOSITORIES: biostudies-literature
Kumar Ramasamy P RP Ranaghan Matthew J MJ Ganjei Allen Y AY Oprian Daniel D DD
Biochemistry 20151203 49
Recoverin (Rv), a small Ca(2+)-binding protein that inhibits rhodopsin kinase (RK), has four EF hands, two of which are functional (EF2 and EF3). Activation requires Ca(2+) in both EF hands, but crystal structures have never been observed with Ca(2+) ions in both sites; all previous structures have Ca(2+) bound to only EF3. We suspected that this was due to an intermolecular crystal contact between T80 and a surface glutamate (E153) that precluded coordination of a Ca(2+) ion in EF2. We construc ...[more]