Ontology highlight
ABSTRACT:
SUBMITTER: Kumar R
PROVIDER: S-EPMC4729746 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Kumar Ravi R Koul Shikha S Kumar Prasun P Kalia Vipin Chandra VC
Indian journal of microbiology 20160120 1
Bacterial identification using rrs (16S rRNA) gene is widely reported. Bacteria possessing multiple copies of rrs lead to overestimation of its diversity. Staphylococcus genomes carries 5-6 copies of rrs showing high similarity in their nucleotide sequences, which lead to ambiguous results. The genomes of 31 strains of Staphylococcus representing 7 species were searched for the presence of common genes. In silico digestion of 34 common genes using 10 restriction endonucleases (REs) lead to selec ...[more]