Ontology highlight
ABSTRACT:
SUBMITTER: Kimmerling RJ
PROVIDER: S-EPMC4729820 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
Kimmerling Robert J RJ Lee Szeto Gregory G Li Jennifer W JW Genshaft Alex S AS Kazer Samuel W SW Payer Kristofor R KR de Riba Borrajo Jacob J Blainey Paul C PC Irvine Darrell J DJ Shalek Alex K AK Manalis Scott R SR
Nature communications 20160106
We introduce a microfluidic platform that enables off-chip single-cell RNA-seq after multi-generational lineage tracking under controlled culture conditions. We use this platform to generate whole-transcriptome profiles of primary, activated murine CD8+ T-cell and lymphocytic leukemia cell line lineages. Here we report that both cell types have greater intra- than inter-lineage transcriptional similarity. For CD8+ T-cells, genes with functional annotation relating to lymphocyte differentiation a ...[more]