Ontology highlight
ABSTRACT:
SUBMITTER: Sarma M
PROVIDER: S-EPMC6459606 | biostudies-literature | 2019 Mar
REPOSITORIES: biostudies-literature
Lab on a chip 20190301 7
Microfluidic devices provide a low-input and efficient platform for single-cell RNA-seq (scRNA-seq). Existing microfluidic devices have a complicated multi-chambered structure for handling the multi-step process involved in RNA-seq and dilution between steps is used to negate the inhibitory effects among reagents. This makes the device difficult to fabricate and operate. Here we present microfluidic diffusion-based RNA-seq (MID-RNA-seq) for conducting scRNA-seq with a diffusion-based reagent swa ...[more]