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Structure of the repulsive guidance molecule (RGM)-neogenin signaling hub.

ABSTRACT: Repulsive guidance molecule family members (RGMs) control fundamental and diverse cellular processes, including motility and adhesion, immune cell regulation, and systemic iron metabolism. However, it is not known how RGMs initiate signaling through their common cell-surface receptor, neogenin (NEO1). Here, we present crystal structures of the NEO1 RGM-binding region and its complex with human RGMB (also called dragon). The RGMB structure reveals a previously unknown protein fold and a functionally important autocatalytic cleavage mechanism and provides a framework to explain numerous disease-linked mutations in RGMs. In the complex, two RGMB ectodomains conformationally stabilize the juxtamembrane regions of two NEO1 receptors in a pH-dependent manner. We demonstrate that all RGM-NEO1 complexes share this architecture, which therefore represents the core of multiple signaling pathways.

SUBMITTER: Bell CH

PROVIDER: S-EPMC4730555 | biostudies-literature | 2013 Jul

REPOSITORIES: biostudies-literature

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Structure of the repulsive guidance molecule (RGM)-neogenin signaling hub.

Bell Christian H CH Healey Eleanor E van Erp Susan S Bishop Benjamin B Tang Chenxiang C Gilbert Robert J C RJC Aricescu A Radu AR Pasterkamp R Jeroen RJ Siebold Christian C

Science (New York, N.Y.) 20130606 6141

Repulsive guidance molecule family members (RGMs) control fundamental and diverse cellular processes, including motility and adhesion, immune cell regulation, and systemic iron metabolism. However, it is not known how RGMs initiate signaling through their common cell-surface receptor, neogenin (NEO1). Here, we present crystal structures of the NEO1 RGM-binding region and its complex with human RGMB (also called dragon). The RGMB structure reveals a previously unknown protein fold and a functiona ...[more]

PMID: 23744777

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