Ontology highlight
ABSTRACT:
SUBMITTER: Aitha M
PROVIDER: S-EPMC4733638 | biostudies-literature | 2015 Apr
REPOSITORIES: biostudies-literature
Aitha Mahesh M Moritz Lindsay L Sahu Indra D ID Sanyurah Omar O Roche Zahilyn Z McCarrick Robert R Lorigan Gary A GA Bennett Brian B Crowder Michael W MW
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry 20150210 3
Previous crystallographic and mutagenesis studies have implicated the role of a position-conserved hairpin loop in the metallo-β-lactamases in substrate binding and catalysis. In an effort to probe the motion of that loop during catalysis, rapid-freeze-quench double electron-electron resonance (RFQ-DEER) spectroscopy was used to interrogate metallo-β-lactamase CcrA, which had a spin label at position 49 on the loop and spin labels (at positions 82, 126, or 233) 20-35 Å away from residue 49, duri ...[more]