Ontology highlight
ABSTRACT:
SUBMITTER: McAndrew CP
PROVIDER: S-EPMC4733873 | biostudies-literature | 2016 Jan
REPOSITORIES: biostudies-literature
McAndrew Christopher P CP Tyson Christopher C Zischkau Joseph J Mehl Patrick P Tuma Pamela L PL Pegg Ian L IL Sarkar Abhijit A
BioTechniques 20160101 1
We report the development of a simple-to-implement magnetic force transducer that can apply a wide range of piconewton (pN) scale forces on single DNA molecules and DNA-protein complexes in the horizontal plane. The resulting low-noise force-extension data enable very high-resolution detection of changes in the DNA tether's extension: ~0.05 pN in force and <10 nm change in extension. We have also verified that we can manipulate DNA in near equilibrium conditions through the wide range of forces ...[more]