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Silver Nanostructures for Fluorescence Correlation Spectroscopy: Reduced Volumes and Increased Signal Intensities.

ABSTRACT: Fluorescence correlation spectroscopy (FCS) is a widely used technique to investigate the interactions and dynamics of molecules, below micromolar concentrations. Silver nanostructure (AgNS) substrates can extend the applicability of FCS to higher concentrations, which is useful for many biologically relevant reactions. Additionally, these substrates can improve detection efficiency by increasing fluorescence signal intensities. The ease of preparation of the AgNS substrates in comparison to previously investigated materials prepared by top-down nanofabrication is expected to make them readily available and suitable for various FCS applications.

SUBMITTER: Choudhury SD

PROVIDER: S-EPMC4743895 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature

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Silver Nanostructures for Fluorescence Correlation Spectroscopy: Reduced Volumes and Increased Signal Intensities.

Choudhury Sharmistha Dutta SD Ray Krishanu K Lakowicz Joseph R JR

The journal of physical chemistry letters 20120920 19

Fluorescence correlation spectroscopy (FCS) is a widely used technique to investigate the interactions and dynamics of molecules, below micromolar concentrations. Silver nanostructure (AgNS) substrates can extend the applicability of FCS to higher concentrations, which is useful for many biologically relevant reactions. Additionally, these substrates can improve detection efficiency by increasing fluorescence signal intensities. The ease of preparation of the AgNS substrates in comparison to pre ...[more]

PMID: 26855696

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