Single-particle electron microscopy in the study of membrane protein structure.

ABSTRACT: Single-particle electron microscopy (EM) provides the great advantage that protein structure can be studied without the need to grow crystals. However, due to technical limitations, this approach played only a minor role in the study of membrane protein structure. This situation has recently changed dramatically with the introduction of direct electron detection device cameras, which allow images of unprecedented quality to be recorded, also making software algorithms, such as three-dimensional classification and structure refinement, much more powerful. The enhanced potential of single-particle EM was impressively demonstrated by delivering the first long-sought atomic model of a member of the biomedically important transient receptor potential channel family. Structures of several more m

SUBMITTER: De Zorzi R 

PROVIDER: S-EPMC4749050 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature