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Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy.

ABSTRACT: Recent advances in technology have enabled single-particle electron microscopy (EM) to rapidly progress as a preferred tool to study protein assemblies. Newly developed materials and methods present viable alternatives to traditional EM specimen preparation. Improved lipid monolayer purification reagents offer considerable flexibility, while ultrathin silicon nitride films provide superior imaging properties to the structural study of protein complexes. Here, we describe the steps for combining monolayer purification with silicon nitride microchips to create a tunable approach for the EM community.

SUBMITTER: Gilmore BL

PROVIDER: S-EPMC5947846 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Preparation of Tunable Microchips to Visualize Native Protein Complexes for Single-Particle Electron Microscopy.

Gilmore Brian L BL Varano A Cameron AC Dearnaley William W Liang Yanping Y Marcinkowski Bridget C BC Dukes Madeline J MJ Kelly Deborah F DF

Methods in molecular biology (Clifton, N.J.) 20180101

Recent advances in technology have enabled single-particle electron microscopy (EM) to rapidly progress as a preferred tool to study protein assemblies. Newly developed materials and methods present viable alternatives to traditional EM specimen preparation. Improved lipid monolayer purification reagents offer considerable flexibility, while ultrathin silicon nitride films provide superior imaging properties to the structural study of protein complexes. Here, we describe the steps for combining ...[more]

PMID: 29605907

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