Ontology highlight
ABSTRACT:
SUBMITTER: Gilmore BL
PROVIDER: S-EPMC5947846 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Gilmore Brian L BL Varano A Cameron AC Dearnaley William W Liang Yanping Y Marcinkowski Bridget C BC Dukes Madeline J MJ Kelly Deborah F DF
Methods in molecular biology (Clifton, N.J.) 20180101
Recent advances in technology have enabled single-particle electron microscopy (EM) to rapidly progress as a preferred tool to study protein assemblies. Newly developed materials and methods present viable alternatives to traditional EM specimen preparation. Improved lipid monolayer purification reagents offer considerable flexibility, while ultrathin silicon nitride films provide superior imaging properties to the structural study of protein complexes. Here, we describe the steps for combining ...[more]