Ontology highlight
ABSTRACT:
SUBMITTER: Wissner RF
PROVIDER: S-EPMC4755331 | biostudies-literature | 2013 Nov
REPOSITORIES: biostudies-literature
Wissner Rebecca F RF Wagner Anne M AM Warner John B JB Petersson E James EJ
Synlett : accounts and rapid communications in synthetic organic chemistry 20130920 18
We have shown that thioamides can be incorporated into proteins through semi-synthesis and used as probes to monitor structural changes. To date, our methods have required the presence of a cysteine at the peptide ligation site, which may not be present in the native peptide sequence. Here, we present a strategy for the semi-synthesis of thioproteins using homocysteine as a ligation point with subsequent masking as methionine, making the ligation "traceless." ...[more]